As expected, ALDHhigh melanoma cells express significantly higher levels of ALDH1A3, SOX2 and CD271 than ALDHlow cells by real time PCR (Figure 4B), western blotting (SOX2/ALDH1A1, Supplementary Figure 4B) and FACS analysis (CD271, Supplementary Figure 4C). This evidence concerns the gene ALDH1A1 and melanoma.