NOS3 and myocardial ischemia: Finally, since eNOS expression and activity is regulated by the insulin signaling pathway (PI3K/p-AktSer473) and is impaired in myocardial ischemia, we investigated whether the restoration of insulin sensitivity by fenofibrate had an effect on the expression of eNOS and its phosphorylated isoform (p-eNOSSer1177).