The expression levels of Nox4, P-p38, cbfα1 and ALP were significantly decreased when pretreated with NADPH oxidase inhibitor DPI, but no significant changes in the expression of RAGE, indicating that CML/RAGE signal was the upstream signals of Nox-derived ROS, p38MAPK, cbfα1 and ALP. Here, NOX4 is linked to chronic myelogenous leukemia, BCR-ABL1 positive.