To further confirm whether caspase-dependent apoptosis plays a prominent role in ZGDHu-1-induced apoptosis, we treated MCL cells with ZGDHu-1 in the presence of the pan-caspase inhibitor Z-VAD.fmk (200 mM) and observed that ZGDHu-1-induced Annexin V+ PI- apoptosis, ZGDHu-1-induced caspase-3 activation as well as the cleavage of PARP was largely abrogated (Figure 3B–3C,Figure 3F–3H and Supplementary Figure 3A, 3C). The gene discussed is CASP3; the disease is mantle cell lymphoma.