Pathway analysis revealed aberrantly expressed mRNAs involved in up-regulated pathways, including cell cycle, focal adhesion, ECM-receptor interaction, pathways in cancer, and PI3K-Akt signaling (Fig 2C), whereas down-regulated pathways included metabolic pathways, glycosphingolipid biosynthesis, drug metabolism, chemical carcinogenesis, and xenobiotics metabolism (Fig 2D). The gene discussed is AKT1; the disease is cancer.