MAPK3 and non-small cell lung carcinoma: To investigate the effect of 2-DG on ERK phosphorylation in NSCLC cell lines of diverse genetic background (Table 1), a panel of cell lines was treated with 25 mM 2-DG for 2 hours, and the phosphorylation of ERK1/2 at Thr202/Tyr204 was examined with Western blot.