PRKAA1 and cancer: To further evaluate the impact of various K-Ras mutations on 2-DG—induced LKB1/AMPK activation, HCT116 cells with only WT endogenous K-Ras were transfected with different cancer-derived K-Ras mutants, including 13D, 12V and 12D, and then were treated with 25 mM 2-DG for 2 hours.