To investigate the significance of the interaction between RanBP9 and TSSC3, we generated SaOS2, U2OS and MG63 osteosarcoma cell lines that stably overexpressed RanBP9 or TSSC3 (RanBP9over or TSSC3over) or stably knocked down RanBP9 or TSSC3 (RanBP9si or TSSC3si), as well as the corresponding control cells (NCover or NCsi). The gene discussed is RANBP9; the disease is osteosarcoma.