We performed immunohistochemical staining of human osteosarcoma tissues to investigate the relevance of RanBP9 and TSSC3 in vivo, and found that expressions of both RanBP9 and TSSC3 (Supplementary Figure S4) were significantly associated with lung metastasis, local recurrence and Enneking stage, but not age, sex, primary location, tumor size and pathological subtype (Table 1). Here, PHLDA2 is linked to osteosarcoma.