In mice without enteritis, there was no effect (P ≥ 0.100) of diet on the expression of Th1 (Il-1β, Ifnγ, Tnfα) or Th17 (Il-17A, Il-22, Il-23A) associated cytokines, Treg (Il-10, Tgfβ), mucus associated (Muc2, Tff3, Relmβ), or bacterial recognition (Tlr2, Tlr4, Myd88, RegIIIγ) genes (Additional file 1: Figure S5). This evidence concerns the gene TNF and enteritis.