The methylation status of 18 candidates, including five genes (RASSF1A, RUNX3, APC, COX2 and CDKN2A) and nine miRNAs (miR-10b, miR-124-3, miR-129-2, miR-203, miR-335, miR-339B, miR-589, miR-647 and miR-671), was examined in 20 HCC tissue pairs (Figure 2). The gene discussed is APC; the disease is hepatocellular carcinoma.