In the present study, we demonstrated that swimming prevented intense oxidative stress and boosted antioxidant status in rats with DSS-induced colitis, as evidenced by reductions in ROS, MDA, and NO levels, iNOS activity, and NF-κB activation, in addition to the reinstatement of GSH and TAC levels and SOD and GPx activity. The gene discussed is NFKB1; the disease is colitis.