To examine the correlation of RUNX3 expression in oral cancer cells with tumor growth and cancer-induced bone destruction, we first established stable RUNX3-knockdown (shRUNX3) Ca9.22 cells and its control (shCTRL) cells by transducing lentiviral particles with RUNX3 and non-specific short hairpin RNAs, respectively. Here, RUNX3 is linked to lip and oral cavity carcinoma.