In the remainder of the melanoma cell lines this effect was not observed, although in some cases the melanoma cell lines did have a relatively high initial level of EBF3. Moreover, in the cell lines where the knockdown led to a reduction in migration or invasion, EBF3 knockdown was also associated with reduced MTT values (WM115, WM266-4, NZM6, NZM9, NZM40, refer to Figure 5). The gene discussed is EBF3; the disease is melanoma.