Mirroring the cell lines, significantly higher expression of EBF3 mRNA (P-value = 7.427e–05, Mann Whitney U test, Figure 5E) was observed in metastatic tumors (median normalized count = 31.63, mean count = 156) compared to primary melanomas (median normalized count = 17.87, mean = 55.16). This evidence concerns the gene EBF3 and metastatic neoplasm.