To investigate whether the elevated EBF3 expression was functionally significant in melanoma cells, we knocked down EBF3 using siRNAs in matched primary/metastatic cell lines (WM115, Hs688(A).T, WM266-4, Hs688(B).T) and in four additional NZM metastatic melanoma cell lines (NZM6, NZM9 NZM11, NZM40), which were included to facilitate the investigations of EBF3 function. The gene discussed is EBF3; the disease is melanoma.