Known tumor suppressor genes were upregulated after transfection of mimetic miR-654-3p, including CASP8, PTEN, DAPK1, RB1 and the cell cycle regulator CDKN2A. Moreover, key genes involved in cell adhesion, migration and ECM remodeling were downregulated after transfection of mimetic miR-654-3p, such as LAMB1 and MMP9. Also, decreased levels of HRAS, FGF2, FGFR4 and IL1B, as well as increased NF1, components of MAPK signal transduction, were observed after transfection of miR-654-3p. The gene discussed is CDKN2A; the disease is neoplasm.