In this study we present the expression pattern of five SPP1 isoforms in low and high grade gliomas, five glioma cell lines and non-transformed astrocytes, and transcriptional regulation of SPP1 by stemness transcription factors GLI1 and OCT4, expressed in glioblastoma cells, but not in normal astrocytes. The gene discussed is POU5F1; the disease is glioma.