Reducing the concentration of fetal bovine serum (FBS) in the growth medium is known to stimulate apoptosis in neuroblastoma cells43,44 and has also been shown to induce an exaggerated response in Myc overexpressing cells.45 Peak levels of apoptosis were achieved after 48 h of exposure to media containing 0.1% FBS; the SH-N-Myc cells showed ~50% apoptosis as measured by Annexin V positivity, which was a twofold increase when compared with SH cells (Figure 6a). The gene discussed is ANXA5; the disease is neuroblastoma.