As shown in Figure 5A and B, matrine significantly inhibited the phosphorylation of Akt, mTOR, p70S6K and 4EBP1 in a dose‐dependent manner in AML cell lines HL‐60, THP‐1 and C1498 as well as primary AML cells. The gene discussed is AKT1; the disease is acute myeloid leukemia.