Later, proteomic analysis of PaCa44 pancreatic adenocarcinoma cell line treated with a chemoterapeutic agent, 5-aza-2′-deoxycytidine (DAC), revealed the 22-fold decreased expression of COTL-1 along with silence of cofilin and profilin 1 [197]. This evidence concerns the gene COTL1 and pancreatic adenocarcinoma.