Utilizing statistical mixed effects regression models, it was determined that the overall slope of the tumor growth regression line was reduced in the MIP3α-gp100 vaccinated group compared to the antigen-only vaccinated group by 48% (p = 0.029), to the irrelevant-antigen group by 56% (p < 0.001), and to the mock vaccine group by 63% (p < 0.001), whereas mock, antigen-only, and irrelevant-antigen vaccines showed no significant differences to each other in slope (Fig. 2a). This evidence concerns the gene PMEL and neoplasm.