So, to avoid all these variances affect our study results, we recruited 39 patients with ischemic cerebral infarct due to large artery occlusion in the MCA territories in subacute stage; none of them had the chance to receive thrombolytic therapy as early treatment of ischemic stroke, and this was intended to avoid good prognostic effect of tPA on clinical outcome. The gene discussed is PLAT; the disease is ischemic stroke.