The co-treatment with the specific intracellular Ca2+ chelator BAPTA-AM evidenced that Ca2+ signalling was responsible for 12(S)-HETE-induced Ser19-MLC2 phosphorylation (Fig. 3b) and furthermore, BAPTA-AM inhibited CRC-induced CCID formation in CT5.3 (Fig. 3c) and CAF3 (Fig. 3d). The gene discussed is MYL2; the disease is colorectal carcinoma.