Additionally, as SV40 co-precipitates poorly with the truncated EMC1 (1-961)-FLAG construct when compared to WT EMC1-FLAG during infection (Figure 5C, top panel, compare lane 2 to 1; quantified in the bottom bar graph), the D961 residue must nonetheless act in the context of the rest of EMC1’s transmembrane domain to fully support virus interaction. The gene discussed is EMC1; the disease is infection.