Immunofluorescence of U-118 MG cultured within the 3D scaffold highlights expression of such CSPG molecules, with specific staining for the molecule brevican, a member of the lectican family (Yiu and He, 2006b), indicating that the glioma cell line expresses CSPGs when cultured within the 3D environment. The gene discussed is BCAN; the disease is central nervous system cancer.