The present study aimed to investigate whether SPN can prevent hypoxia-induced retinal neovascularization via inhibition of HIF-1α/VEGF signaling pathway in a VEGFA secretion model of ARPE-19 cells induced by CoCl2 in vitro and a neovascularization rat model of oxygen-induced retinopathy in vivo. This evidence concerns the gene HIF1A and retinal disorder.