RPS6 and hepatocellular carcinoma: Rather than reconstituting PIC assembly with purified components, we incubated the RNA in lysate from Huh 7.5 (human hepatoma) cells to replicate cellular conditions and then used antibodies to detect bound 43S complex components eIFs 3, 2, 1A, 1 and 40S subunit (protein rpS6) (Figure 1A).