IGFBP5 and ovarian cancer: Adenovirus expressing IGFBP-5 was prepared and used to infect the MDAH 2774 (hereafter referred to as 2774) ovarian cancer cell line, which expresses very low levels of IGFBP5. Adenoviral IGFBP-5 expression was confirmed by western blotting using an anti-Myc antibody (Fig. 1D, upper panel).