AXL and neoplasm: Figure 4d shows untreated and Supplementary Fig. 4A shows IFN-γ treated cells changes in Axl expression by flow cytometry in wild-type Py8119 cells, vector control Py8119 cells, Py117 cells and five Py8119 Axl CRISPR tumour cell clones (Axl Cr#1–5). Western blot analysis confirmed elevated expression of Axl protein in Py8119 cells as well as higher levels of Tyr702 phosphorylation. In contrast, complete knockout of Axl in the CRISPR clones resulted in undetectable levels of Axl protein and Tyr702 phosphorylation (Fig. 4e).