To incorporate the effect of a differential in vivo antigen uptake and processing capacity in our assay, TADCs were sorted from LLC-OVA tumours and immediately co-cultured with carboxyfluorescein succinimidyl ester (CFSE)-labelled TCR transgenic CD8+ OT-I T cells or CD4+ OT-II T cells without additional ex vivo Ag loading or stimulation. The gene discussed is CD8A; the disease is neoplasm.