CD274 and neoplasm: We next compared the T-cell activating capacity of tumour-associated cDC1s and cDC2s with cDC1 and cDC2 subsets from naive spleens (gating strategy in Supplementary Fig. 6A), which also expressed high levels of the costimulatory molecules CD80, CD86, PDL1 and PDL2 (Supplementary Fig. 6B).