Confining the time point of serological evaluation to the active and late phase of ZIKV infection, i.e. ≥ 6 days after symptom onset, anti-ZIKV IgM reactivity was observed in 10/17 (58.8%) patients with positive ZIKV-RT-PCR and 3/38 (7.9%) patients with suspected ZIKV infection, while anti-ZIKV IgG was detectable in 15/17 (88.2%) and 34/38 (89.5%) cases, respectively. This evidence concerns the gene CD40LG and Zika virus infectious disease.