Indeed, miR-4286 inhibition resulted in degradation of folylpolyglutamate synthase, RNA polymerase I-specific transcription initiation factor, apelin, G-protein-coupled receptor 55, and high-mobility group A1 protein mRNA in BRO melanoma cells, as measured by real-time PCR (Fig 7, S9 Table). Here, APLN is linked to melanoma.