SNAI2 and neoplasm: Conversely, loss-of-function studies using CL1–2 cells, in which endogenous Daxx or both Daxx and Slug were knocked down showed that, whereas mice in the control group (shCtrl) did not form nodules in orthotopic lung tumour assays, 44% of mice (four of nine) in the Daxx-knockdown group (shDaxx) formed primary lung tumours and 50% of these primary tumours (two of four) formed metastatic lung nodules (Fig. 5f; Supplementary Fig. 8a,b).