RNA fluorescence in situ hybridization (RNA-FISH) was performed using LNA probes spanning the exon–exon junctions 3–4 and 4–5, respectively, and showed SNHG5 to be predominantly cytosolic in both HCT116 CRC cells and in immortalized TIG-3 human fibroblasts (Fig. 1d). Here, SNHG5 is linked to colorectal carcinoma.