In brain tumors, CAR studies targeting six different antigens (EGFR, EGFRvIII, EphA2, Her2, IL13Rα, and MUC1) are ongoing (NCT02331693, NCT01454596, NCT02575261, NCT02442297, NCT02208362, NCT02617134), using second (CD28 or 41BB costimulation) or third (CD28 and 41BB costimulation) generation constructs (Table 2). This evidence concerns the gene CD28 and brain neoplasm.