Consistent with the initial ISG screen, a dose-response to infection revealed that MYD88 and UNC93B1 reduced Lm infectivity (defined as the MOI of Lm required to achieve 50% cellular infection during the course of 8 hours) by 9.7-fold and 5.7-fold compared to firefly luciferase control (Fig 3A). Here, UNC93B1 is linked to infection.