The experiments from this study revealed that there could be negative feedback in colon cancer cells where NHERF1 up-regulation was induced by hypoxia, depending on the activation of VEGFR2 signaling pathway, in turn reducing the phosphorylation promoted activation of VEGFR2 signaling, resulting in the inhibition of migration and invasion of colon cancer cells. This evidence concerns the gene KDR and colonic neoplasm.