In the present study, in vitro functional experiments showed that overexpression of NHERF1 reduced cell migration and invasion of RKO cells, and knockdown expression of NHERF1 enhanced the migratory and invasive ability of RKO cells, indicating a tumor suppressor role of NHERF1 in colon cancer cells specifically for metastasis (Figure 3). Here, NHERF1 is linked to colonic neoplasm.