The estimate was very similar after excluding potentially pleiotropic SNPs but this association was not robust to the MR-Egger method which gave much less weight to the SNP (rs2954021) from PNPLA3. Genetically predicted ALP was not clearly associated with T2DM using IVW (see Supplementary Fig. S2a). This evidence concerns the gene PNPLA3 and type 2 diabetes mellitus.