Among these miRNAs, miR-182 was previously reported to be involved in cancer metastasis and TGFβ signalling by us and others19, 23, and thus represented a promising candidate to interfere TGFβ regulation of SMAD7. We cloned SMAD7 3′UTR sequence containing the predicted miR-182 binding site for luciferase reporter assays. Here, TGFB1 is linked to cancer.