BRAF and Diarrhea: NS7 gene-specific primers (NS7-F: 5′-GCTGAATTCATGGAGTTC CGCTTAACTCCGCCAT-3′; NS7-R: 5′-CATCTCGAGCTAGAGCCATGATGCGAGGATCAG-3′) were designed based on the sequence of the PDCoV strain CHN-HN-2014 (GenBank accession number KT336560), which was isolated from piglets with severe diarrhoea on a pig farm in Henan Province, China [24].