In cells with reduced levels of ACE, Ang-II was unable to induce TM-5 cell proliferation (Control: 147 ± 23%, siRNA SCR: 172 ± 9% and siRNA ACE: 0 ± 2%, n = 4, p<0.05), (Fig 6B), indicating that ACE activation is involved in melanoma proliferative response triggered by Ang-II. This evidence concerns the gene ACE and melanoma.