To further explore whether Gankyrin could induce EMT through p-STAT3/ p-SMAD3 or p-SMAD3/p-STAT3 in NSCLC, we applied siRNA transduction to knockdown the STAT3 and SMAD3 expression in Sk-lu-1 cell lines, respectively, then detected the EMT-related biomarkers and p-AKT, p-STAT3 and p-SMAD3 expressions by Western blot assay. The gene discussed is SMAD3; the disease is non-small cell lung carcinoma.