Withthis approach, we showed that a combined positive (from PKB [11,12]) and negative(from S6K [12,13]) cross-talk to mTORC2 in the insulin-signalling network canaccurately describe the previously unexplained increased PKB-S473P in response toinsulin in insulin-resistant human adipocytes from patients with Type 2 diabetes(Figure 6). The gene discussed is RPS6KB1; the disease is type 2 diabetes mellitus.