It is noteworthy that MLN-4924 inhibited basal non-cytokine-mediated HCVpp infection (Fig. 4c), whereas it had no effect on the permissiveness of non-polarized HepG2 or Huh-7 cells to support HCVpp entry, suggesting a role for NF-κB signalling in polarized HCV entry. This evidence concerns the gene NFKB1 and infection.