CASP3 and steatosis: Our findings are comparable to those of Moravcova et al., who found 1 mM fatty acid mix (palmitate: oleate acid, 2:1) treatment increased triglyceride, ROS, caspase 3 and reduced mitochondrial membrane potential (Δψm) in primary liver cells, and further recommended in vitro models as suitable tools for studying hepatocellular consequences of steatosis [22].