TLR8 and obesity due to melanocortin 4 receptor deficiency: Besides, the present study is also limited by certain factors as follows: (1) small cohort size for each BMI group; (2) the lack of data expressing functional changes in relation to altered TLR8 expression in the adipose tissue; and (3) the data represent TLR8 global changes in the adipose tissue in obesity/T2D and hence selective expression of TLR8 in adipocytes and macrophages remains unknown.