TP53 and malignant colon neoplasm: We first performed gene targeting of wild-type (WT) TP53 in the human HepG2 hepatoma cell line and generated isogenic cell clones harboring exon 2 deletions of TP53 to induce endogenous Δ40p53 expression using adeno-associated virus (AAV)-based methodology (Fig. S1A), as previously observed in colon cancer HCT116 TP53−/− cell clones previously (Thomas et al., 2013).