In order to assess the suppressive ability of the above mentioned Treg subsets in NSCLC patients and HDs, isolated enriched Treg of all stages of activation and differentiation (CD127−/lowCD25+CD4+) were co-cultured with activated CD4+ T-cells and culture supernatants were tested for IFNγ production. Here, CD4 is linked to non-small cell lung carcinoma.