However, we did not find that daunorubicin significantly activated NF-κB at the basal level or further after induction with TNFα and this could be due to differences in concentrations used as well as cell types; we used 100 nM daunorubicin in OCI-AML3 cells and Das & White used from 2-5 μM in the human lung adenocarcinoma cell line A549 [42]. The gene discussed is TNF; the disease is lung adenocarcinoma.