We therefore sought to identify long synthetic peptides (20mers or longer) that had the potential to be truly universal HLA-DR (MHC class II) binders, based on cleavable embedded 15mer peptide sequences [37], as well as cleavable embedded 8-9mers with high avidity for the widely prevalent MHC Class I haplotype HLA-A2.1 (see Materials and Methods), We applied this strategy to predict immunogenic “hot spots” within the widely prevalent tumor-associated proteins MUC1 and HER2 (Figure 3 and Figure 4), as well as the brain tumor-associated pp65 cytomegalovirus (CMV) protein [21, 38–41]. Here, MUC1 is linked to brain neoplasm.