We detected an FGF6 mutation and four potentially actionable FGFR1 alterations, including one FGFR1 missense mutation, as well as three FGFR1 fusions including a novel FGFR1–HOOK3 fusion and two cases with an FGFR1–TACC1 fusion previously reported in glioblastoma multiforme. This evidence concerns the gene HOOK3 and glioblastoma.