Comparison of the effects of CAP1, CAP2 and CAP3 on CC14 primary human colon cancer cells [6,8,10,11] showed the higher potency of CAP2, which is the aglycone of CAP1 (Fig 1A): Analysis of fluorescence intensity of CC14-GFP+ cells, as a measure of overall cell mass and viability, treated with CAP1, CAP2, CAP3 or DMSO-only as control revealed that at 2.5μM all three CAP molecules had similar negative effects (panel C in S3 Fig). This evidence concerns the gene CAP2 and colonic neoplasm.