This included Ls174T cells harboring a gain of function ßCATENIN mutation, DLD1 and HT29 cells with loss of function mutations in APC (Fig 1C and not shown), as well as in three not cloned primary colon cancer cells: two from bowel tumors (CC14 and CC36) and one from a liver metastasis (mCC11) [6,8]. Here, APC is linked to malignant colon neoplasm.