Before studying the influence of PLAT gene promoter/enhancer methylation on PLAT gene expression, we investigated the PLAT transcriptional start site (TSS) by 5’RACE analysis using total RNA from five t-PA expressing cell types: astrocytes, Bowes melanoma cells, HeLa cells, HUVEC and HT1080 cells (Fig 1). This evidence concerns the gene PLAT and melanoma.